Fig. 2. S-allylcysteine (SAC) suppressed dose-dependently the proliferation of HNE1 and HONE1 cell lines. SAC inhibited colony forming ability induced by hepatocyte growth factor in HNE1 cell line. (A) MTT assay was performed at 24, 48, and 72 hours in HNE1 and HONE1 cells. (B) Soft agar assay in which cells were seeded at a density of 2.5 × 10³ cells/mL and cultured in 0.4% fetal bovine serum at 37℃ for 21 days in HNE1. SAC was added to the soft agar every 3 days. After 3 weeks, the colonies were stained with 0.05% crystal violet. The stained colonies were counted using MetaMorph NX image software. Data shown represent the mean ± standard deviation. MTT, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium; NS, not significant. ^*p < 0.05, ^***p < 0.001.