Fig. 4.
Mitotic clonal expansion of BMP4-committed C3H10T1/2 stem cells upon induction with differentiation inducers. (A) C3H10T1/2 stem cells were cultured with or without 50 ng/ml BMP4 until postconfluence and then induced to differentiate with 3T3-L1 standard differentiation protocol. Cells were counted on day 6 and plotted. (B) Committed C3H10T1/2 (by BMP4 at 50 ng/ml) stem cells and 3T3-L1 and 3T3-F442A preadipocytes were induced to differentiate, and the expression level of cyclin A was followed by Western blotting.