Table 1.
List of primer sequences and PCR conditions used for TNF- α gene polymorphisms.
| SNPs | rsID | Primers | Annealing (°C) | Amplicon Size (bp) |
|---|---|---|---|---|
| − 308G/A | rs1800629 | F.P- 5-AGGCAATAGGTTTTGAGGGCCAT-3 R.P- 5-TCCTCCCTGCTCCGATTCCG-3 S.P- 5- CAATAGGTTTTGAGGGGCATG -3 |
55 | 107 |
| + 488G/A | rs1800610 | F.P- 5-GCCAGACATCCTGTCTCTCC-3 R.P- 5-CAGAGGGAAGAGGTGAGTGC-3 S.P – 5- TGCATCCCCGTCTTTCTCCA -3 |
60 | 220 |
| − 857C/T | rs1799724 | F.P- 5-GGCTCTGAGGAATGGGTTAC-3 R.P- 5-CCTCTACATGGCCCTGTCTAC-3 S.P- 5- GTATGGGGACCCCCCCTTAA -3 |
56.5 | 127 |
| − 1031 T/C | rs1799964 | F.P- 5-TATGTGATGGACTCACCAGGT-3 R.P- 5-CCTCTACATGGCCCTGTCTT-3 S.P- 5- CAAAGGAGAAGCTGAGAAGA -3 |
63 | 264 |
Abbreviations: F.P-Forward primer; R.P-Reverse Primer; S.P-SNaPshot Primer; bp-base pair.