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. 2016 Jan 11;9:494. doi: 10.3389/fnins.2015.00494

Figure 1.

Figure 1

Retroviral knockdown of DCX does not affect NPC survival and differentiation. (A) Representative images and quantification of NPCs showing no difference in normalized counts between shDCX-GFP cells compared to Ctrl-GFP cells at 12 and 30 dpi; (B) Proportion of GFP+ NPCs expressing DCX was significantly reduced in shDCX-GFP compared to Ctrl-GFP, with some shDCX cells having fainter DCX staining (arrows = GFP+ DCX+; arrowhead = GFP+ DCX−; star = GFP+DCX+ faint). (C) Expression of the immature neuronal marker NeuroD1 is unaffected between control and shDCX expressing cells (arrows = GFP+NeuroD1+ colabeled cells). (D) Neuronal fate was not affected as there was no difference in proportion of GFP+ cells expressing the mature neuronal marker NeuN between control and shDCX at 30 dpi. N = 3–6 mice per group with the total number of cells analyzed in (B) 105 Ctrl and 127 shDCX cells; (C) 151 Ctrl and 201 shDCX cells; (D) 130 Ctrl and 84 shDCX cells. *p < 0.05. Scale bar = 20 μm for (A) and 10 μm for (B–D).