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. 2016 Jan 11;9:506. doi: 10.3389/fncel.2015.00506

Figure 5.

Figure 5

Decreased UCHL1 levels in SMA motor neurons. (A) Representative western blot showing UCHL1 protein levels in three different control and SMA motor neuron cell lines. The graph above it shows the integrated density of the UCHL1 bands from this blot/total protein (Coomassie gel), as determined by ImageJ software. The graph to the right shows the average integrated density of the UCHL1 bands from this blot/total protein (Coomassie gel). Error bars represent standard error from the mean and statistical significance was calculated using an unpaired, one-tailed t-test with two-sample unequal variance. (B) Representative confocal images indicate a reduction of UCHL1 levels in SMI32 positive cells. (C) Average gene expression levels of UCHL1 in the control and SMA motor neuron cell lines [the same six cell lines shown in (A)], as determined by qRT-PCR (p = 0.04; unpaired, one-tailed t-test). Relative fold expression was normalized to H9 hESCs. Error bars represent standard error from the mean. CTR, control, MNs, motor neurons. *p ≤ 0.05.