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. 2015 Dec 17;6:386–393. doi: 10.1016/j.dib.2015.12.003

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Gating strategy. The gating was done according to the colocalization wizard in the IDEAS software. Only cells in focus (A) and single cells (B) were included. Neutrophils were gated based on their positivity of DAPI and being low in side scatter (SSC), excluding anuclear debris and eosinophils (high in side scatter due to inherent aoutofluorescence) (C). Finally, only neutrophils with strong positive staining for both markers were selected (D). Brightfield (BF) images and indicated fluorescently labeled markers are depicted.