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. 2015 Dec 28;2016:9674614. doi: 10.1155/2016/9674614

Figure 4.

Figure 4

Effect of high glucose on adipogenic differentiation of gestational tissue-derived MSCs. (a) Representative micrographs show the morphology of adipocyte-like cells (red colored cells) derived from CH-MSCs, PL-MSCs, and UC-MSCs cultured in adipogenic differentiation medium with or without glucose supplementation for 28 days after staining with oil red O. Scale bar: 50 μm for DMEM, 100 μm for ADIPO, and ADIPO + glucose. (b) Graph shows the number of adipocyte-like cells generated from CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 28 (n = 3). (c) Graph shows the percentages of adipocyte-like cells in total cell number generated from CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 28 (n = 3). (d) Graph shows the concentrations of oil red O staining of CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 28 (n = 3). (e) Graph shows relative mRNA levels of adipogenic genes ADIPOQ, GLUT4, LPL, PPARγ, and SREBP1C of CH-MSCs, PL-MSCs, and UC-MSCs cultured in high glucose condition in comparison to their normal glucose controls on culture day 28 (n = 3). Data were presented as mean ± standard error of the mean (SEM). ∗ P < 0.05 versus ADIPO. n corresponds to the number of independent samples used in the experiments. DMEM: MSCs cultured in complete medium which serve as nondifferentiation controls. ADIPO: MSCs cultured in adipogenic differentiation medium without glucose supplementation which serve as normal glucose controls. ADIPO + glucose: MSCs cultured in adipogenic differentiation medium supplemented with 25 mM D-glucose.