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. 2015 Dec 28;2016:5874127. doi: 10.1155/2016/5874127

Figure 5.

Figure 5

Snail is crucial for the CCL21 promoted cancer stem cell properties. (a) HCT116 cells were transfected with control vector pcDNA-3.1 (Vector) and pcDNA-Snail for 24 h, and the expression of Snail at protein level was detected by western blotting. (b) HCT116 cells were transfected with control vector pcDNA-3.1 (Vector) and pcDNA-Snail for 24 h, and the expression of Snail at mRNA level was detected by qRT-PCR. (c) Control vector pcDNA-3.1 (Vector) and pcDNA-Snail were transfected into HCT116 cells and were detecting the mammosphere forming ability. (d) Control vector pcDNA-3.1 (Vector) and pcDNA-Snail were transfected into HCT116 cells for 24 h, 48 h, and 72 h, and then the expressions of Bmi-1, Nanog, and Oct-4 at protein level were analyzed via western blotting. (e) Control vector pcDNA-3.1 (Vector) and pcDNA-Snail were transfected into HCT116 cells for 24 h, 48 h, and 72 h, and then the expressions of Bmi-1, Nanog, and Oct-4 at mRNA level were analyzed via qRT-PCR.