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. 2004 Jun 21;101(26):9677–9682. doi: 10.1073/pnas.0403250101

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Copyright © 2004, The National Academy of Sciences

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Fig. 1. — Levels of pRB family members in Lmna^-/- cells. In all blots, -/- indicates the Lmna^-/- genotype and +/+ indicates the litter-matched Lmna^+/+ genotype. Other genotypes are as described. (A) pRB levels in mouse embryo fibroblasts. Antibodies to Rho GDP were used as loading control. (B) pRB levels in immortalized fibroblasts. Antibodies to Rho GDP were used as loading control. (C) Equilibrated extracts from immortalized fibroblasts of indicated genotype were immunoprecipitated with antibodies to p107. Immunoprecipitated proteins were analyzed by Western blots with p107-specific antibodies. The lower band is the heavy chain of the p107 antibodies used for immunoprecipitation. (D) p130 levels in immortalized fibroblasts. Flanking bands are nonspecific. (E) p27KIP1 levels in immortalized fibroblasts. (F) Emerin levels in immortalized fibroblasts.