Table 1.
The primers sequences used for detection of IFN-γ (+874A/T), IFN-γR1 (-611A/G), (+189T/G), and (+95C/T) gene polymorphisms using ARMS-PCR and RFLP-PCR.
| Gene | Polymorphisms | Primers | Sequence |
|---|---|---|---|
| IFN-γR1 | -611A/G | Forward | AGAGCAGACCTCTTCATGAGAGGCTGTCT |
| rs1327474 | Reverse | ACATTTTTAGAAGAGAATGAGACTTCAAA | |
| +95C/T | Forward | GCCATTTGGTGGTCCATTAC | |
| rs7749390 | Reverse | TCCAGACAGCTGGAATCAGT | |
| +189T/G | Forward | CTCTTTCTCCTACCCCTTGTCAT | |
| rs11914 | Reverse | CAGCGCATAATCGTATTTAAAAGTG | |
|
| |||
| IFN-γ | +874A/T | Forward (T allele) | TTCTTACAACACAAAATCAAATCT |
| rs62559044 | Forward (A allele) | TTCTTACAACACAAAATCAAATCA | |
| Reverse | TCAACAAAGCTGATACTCCA | ||