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Freshly isolated CD8+ tumor-infiltrating lymphocytes (TILs) showed distinct phenotypical difference compared with CD8+ peripheral blood T cells (PBTCs). Phenotypic characterization of freshly isolated CD8+ T cells in TILs and PBTCs from patient (A) and patient (B). Expression of CCR7 and CD45RO (a) and PD-1, LAG-3, and TIM-3 (b) in CD8-gated live cells in TILs and PBTCs are shown. Number indicates the percentage of cells shown in each quadrant (a) or indicated gated regions (b).
