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. 2015 Dec 28;2016:3615286. doi: 10.1155/2016/3615286

Figure 2.

Figure 2

Hypoxia marker expression. (a) Levels of hypoxia-inducible factor 1 alpha (HIF-1α) mRNA expression after 24 hours in culture under normoxia (PO2 160 mmHg) and hypoxia (PO2 15 mmHg) at 150, 300, and 600 IEQ/cm2. (b) Translocation and activation of HIF-1α protein were performed after 24 hours in each density (150, 300, and 600 IEQ/cm2) under normoxia and hypoxia conditions. (c) Representative images of hypoxyprobe stained cells at 150, 300, and 600 IEQ/cm2 under normoxia and hypoxia. Blue staining highlights the cell nuclei (DAPI), and the green staining indicates pimonidazole (hypoxia marker) expression. Scale bars = 50 μm. ααα p < 0.001 compared to the same concentration under normoxic conditions, & p < 0.05 compared to 150 IEQ/cm2 under hypoxic conditions, and α p < 0.05 compared to the control.