Table 2.
Microsatellite loci tested for PCR amplification in aspen.
| Loci | Primer sequences (5′- 3′) | Repeat motif | Fragment size (bp)3 |
|---|---|---|---|
| ORPM141 | F- GGGCTGCAGCAGATATTGA R- CCAAAGGAACCCAAAGAAGA |
(GCTC)4 | 146–162 |
|
| |||
| ORPM181 | F- AGCAGAGATCGATGCTGAGG R- AATTTCTCGCTTCTCGCATT |
(TTTA)4 | 205 |
|
| |||
| ORPM361 | F- AGCCTCCAAACACCATGAAC R- ACAGTGGTGTGGATCCTGCT |
(GAAA)4 | 213 |
|
| |||
| ORPM601 | F- ATAGCGCCAGAAGCAAAAAC R- AAGCAGAAAGTCGTAGGTTCG |
(AAT)5 | 212 |
|
| |||
| ORPM791 | F- GAAGCTGAAAACAACAACAAACA R- GGGTTTTTAACATAATAAAAGCTTGG |
(AAT)4 | 160 |
|
| |||
| ORPM811 | F- GCTGCAGCCAAACAAAGC R- CAGAAATCCCACCCAAACC |
(TATT)4 | 142–158 |
|
| |||
| ORPM841 | F- CTGCAGCCTTACCACCATTT R- CGTGTTCGAGATTGGGATTT |
(AAG)4 | 171 |
|
| |||
| ORPM861 | F- CCACATCCATAGCTCTGCAAC R- GTACTACCTCGCCTGCCAAC |
(CTT)5 | 204 |
|
| |||
| ORPM1071 | F- AATCTGGTGGCTTGCCTCT R- TTGAGGAACACGTGCAGACT |
(TAAA)4 | 190 |
|
| |||
| ORPM1171 | F- CCCCCTAATTACCTTGGAAAC R- TTGTTTGTATCTCCTCCGTTGA |
(ATTA)4 | 210 |
|
| |||
| ORPM1581 | F- GCTGAAACATCCTTCATGGTC R- CGAAGCTGCATAAGCATCAA |
(TTTC)4 | 200 |
|
| |||
| ORPM1931 | F- CCGCTGGATTTGTTTGTTTT R- TGAGCAGAAAGATGCGAAGA |
(ATTTT)4 | 187–207 |
|
| |||
| ORPM2021 | F- TCGCAAAAGATTCTCCCAGT R- TTCAAATCCCGGTAATGCTC |
(TAA)5 | 184–190 |
|
| |||
| ORPM2061 | F- CCGTGGCCATTGACTCTTTA R- GAACCCATTTGGTGCAAGAT |
(GCT)7 | 190–208 |
|
| |||
| ORPM2201 | F- AGCTAGCCTGTCGTCAAGGA R- CAAGGAAGCATTCTCGCAAT |
(TTTA)6 | 178–222 |
|
| |||
| ORPM2961 | F- CGAAGCCATTGACCCAGTAT R- GGGCATCTCTCTCCTTTCAA |
(GTTCTG)4 | 199 |
|
| |||
| ORPM3121 | F- GTGGGGATCAATCCAAAAGA R- CCCATATCAAACCATTTGAAAAA |
(CCT)6 | 194 |
|
| |||
| ORPM3661 | F- CCTTGAGGGGACACTTCGAT R- AAAGAGTTGAGCCCTTGGTG |
(TTA)5 | 156 |
|
| |||
| ORPM3711 | F- CCGGACTCTCACAAATCTCC R- TGCTTTTGCTCCTGGTTCTT |
(TCTT)6 | 192–200 |
|
| |||
| ORPM3721 | F- AGCTCTTCTGCTGGTGCTGT R- GAGGGAGGGAGGGTAAAAGA |
(TCTT)5 | 190 |
|
| |||
| ORPM4151 | F- CTCGGTGCAAATATCGGTTC R- AGATCGATGGTCCTTTCCTG |
(GGCG)4 | 225 |
|
| |||
| ORPM4841 | F- CAAAATGGCAATCCAAGGTT R- CCAAGCTTCCAATTGAGTCC |
(TTAA)4 | 190–206 |
|
| |||
| ORPM4881 | F- CTCCAGCCGCTTCTATCCTT R- TGTCGTGGGAAAGAACCAGT |
(TTA)6 | 200 |
|
| |||
| ORPM4961 | F- CAGCAGTGCAAGCTCCTAAA R- GGCCACTGACAGAGACCAAG |
(GGA)4 | 185 |
|
| |||
| WPMS142 | F- CAGCCGCAGCCACTGAGAAATC R- GCCTGCTGAGAAGACTGCCTTGAC |
(CGT)28 | 215–287 |
|
| |||
| WPMS152 | F- CAACAAACCATCAATGAAGAAGAC R- AGAGGGTGTTGGGGGTGACTA |
(CCT)14 | 201–219 |
|
| |||
| WPMS162 | F- CTCGTACTATTTCCGATGATGACC R- AGATTATTAGGTGGGCCAAGGACT |
(GTC)8 | 139–184 |
|
| |||
| WPMS172 | F- ACATCCGCCAATGCTTCGGTGTTT R- GTGACGGTGGTGGCGGATTTTCTT |
(CAC)15 | 122–146 |
|
| |||
| WPMS182 | F- CTTCACATAGGACATAGCAGCATC R- CACCAGAGTCATCACCAGTTATTG |
(GTG)13 | 219–248 |
|
| |||
| WPMS192 | F- AGCCACAGCAAATTCAGATGATGC R- CCTGCTGAGAAGACTGCCTTGACA |
(CAG)28 | 180–234 |
|
| |||
| WPMS202 | F- GTGCGCACATCTATGACTATCG R- ATCTTGTAATTCTCCGGGCATCT |
(TTCTGG)8 | 210–222 |
|
| |||
| WPMS212 | F- TGCTGATGCAAAAGATTTAG R- TTGGAACTTCAACATTCAGAT |
(GCT)45 | 287–326 |
|
| |||
| WPMS222 | F- ACATGCTACGTGTTTGGAATG R- ATCGTATGGATGTAATTGTCTTA |
(TGA)23 | 100–135 |