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. 2016 Jan 11;6:1526. doi: 10.3389/fmicb.2015.01526

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Copyright © 2016 Pitangui, Sardi, Voltan, dos Santos, da Silva, da Silva, Souza, Soares, Rodríguez-Arellanes, Taylor, Mendes-Giannini and Fusco-Almeida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comet assay data of AMJ2-C11 macrophages infected with H. capsulatum yeasts. (A) Images were acquired using a fluorescence microscope equipped with a 515–560 nm excitation filter and a 590 nm barrier filter. (B) Evaluation of nuclear fragmentation in AMJ2-C11 macrophages infected with H. capsulatum. Control (non-infected macrophages). Values are representative of the percentage of fragmented DNA present in the comet's tail. (C) Percentage of macrophage population showing DNA damage in infected and non-infected (control) cells. Scores given are the mean ± SD. Data were analyzed by Kruskal-Wallis test with Dunn's post-test. ^*P < 0.05 when control vs. strain EH-315 was compared; #P < 0.05 when control vs. strain 60I was compared.