Figure 2.
NIN Expression in the Root Epidermis Is Sufficient to Induce Nodule Formation.
(A) to (E) Wild-type (A) and nin-1 ([B] to [E]) M. truncatula roots were transformed with A. rhizogenes containing a control vector ([A] and [B]), pNIN:gNIN (C), pUb:gNIN (D), or pEXPA-gNIN (E) and inoculated with S. meliloti (50 d after inoculation). Numbers indicate the number of transformed plants showing nodules out of the total number of transformed plants.
(F) Nodules numbers at 50 DPI in wild-type (WT) and in nin-1 roots transformed with a control vector or pNIN:gNIN, pEXPA:gNIN, or pUb:gNIN. Only plants showing nodules are included in the analysis. Central lines show the medians, crosses show the averages, and the box delimits the 25th and 75th percentiles as determined by R. The whiskers extend 1.5 times the interquartile range from the 25th and 75th percentile, with outliers represented by dots.