Figure 5.

Development of Pollen of the pprd2-1^+/− Line: Effect of Supplementation with Dol.

(A) Expression of PPRD2 in wild-type and pprd2-1^+/− pollen. Values are the means ± sd of three independent experiments.

(B) to (D) Germination of pollen grains from wild-type and heterozygous pprd2-1^+/− plants on regular medium (B), medium supplemented with polyprenol or Dol (C), or with tunicamycin ([D]; final concentration 10 or 50 ng/mL). Tube deformations (arrows), significantly more frequent for pprd2-1^+/− than wild-type plants, are rescued by Dol but not Pren. Tunicamycin significantly increases number of deformed pollen tubes. Bar = 100 μm.

(E) Quantification of the effect of tunicamycin on pollen tube development. Number of pollen tubes viewed is 164, 203, and 241 for control, 10 and 50 ng/mL tunicamycin, respectively (Supplemental Table 5). Means ± sd are shown. Statistically significant differences from the wild type are indicated: *P < 0.05

See also Supplemental Figure 8 and Supplemental Table 5.