Figure 1.

CDC20.1 Mutant Plants Exhibit Reduced Fertility.

(A) Plant growth phenotypes of the wild type and mutants. Short siliques, indicating reduced fertility, are marked by arrowheads in cdc20.1. Bar = 5 cm.

(B) Pollen grain viability in wild-type and mutant anthers as assayed by Alexander Red staining. Anthers of the CDC20.1 mutants show a significant proportion of dead pollen stained green and a few residual viable pollen grains. Bar = 50 μm.

(C) Wild-type pollen grains are of uniform size, while the size of cdc20.1 pollen grains was variable, with larger (black arrows) and smaller (red arrows) grains than those of the wild type. Bar = 25 μm.

(D) Tetrad stage microspores in the wild type and mutants. Wild-type tetrad stage microspores have four equal size members, while cdc20.1 samples at the same stage contain more than four microspores (numbered). Bar = 25 μm.