Figure 4.

Chromosome Orientation Is Defective in cdc20.1-3.

Pachytene (A), diplotene (B), diakinesis (C), metaphase I (D), anaphase I (E), metaphase II (F), and anaphase II (G). Chromosomes are stained with DAPI in blue, and centromeric regions are marked by red fluorescence. The wild type has five paired homologs (bivalent) at diakinesis and the paired centromeres in each bivalent are indicated with numbers (C). The arrows indicate abnormal association at diplotene (B) and diakinesis (C), and improper orientations at metaphase I (D) and metaphase II (F) in cdc20.1-3. Bars = 5 μm.