Table 1. The known functional sites (FSs) and the Rsite2 hits of the tRNA (Lys).

Site No.	Known FS	Rsite2 hit	Description
1	1–4	1	Within acceptor stem(5’ end) Interacting with Ran Contacting Xpot Involved in recognition by RNase Z and RNase P Recognized by aminoacyl-tRNA synthetase
2	13–22	18	DHU loop Interacting with the mRNA-ribosome complex Contacting Xpot Involved in recognition by RNase P
3	34–36	35	Anticodon, Decoding mRNA codon Recognized by aminoacyl-tRNA synthetase Interacting with the mRNA-ribosome complex
4	49–51	49	Within TψC stem Binding site of elongation factor
5	53–61	58	TψC loop Interacting with the mRNA-ribosome complex Contacting Xpot Involved in recognition by RNase Z and RNase P Processed by a tRNA ψ55 pseudouridine synthase Affect 3’ end processing and tRNA structure
6	63–65	-	Within TψC stem Binding site of elongation factor Interacting with Ran Involved in recognition by RNase P
7	72–76	75,76	Aminoacylation site(3’ end) Recognized by aminoacyl-tRNA synthetase Interacting with the mRNA-ribosome complex Contacting Xpot Involved in recognition by RNase Z Processed by a CCA-adding enzyme