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. 2004 Jun 21;101(26):9763–9768. doi: 10.1073/pnas.0403235101

Fig. 2.

Fig. 2.

Surface plasmon resonance analysis of the interaction of human FcRn with EpoFc and human IgG1. Different concentrations of IgG1 or EpoFc fusion proteins (0.004-10 μM) were injected over a flow cell coupled with human FcRn (3,500-5,000 response units) at a flow rate of 10 μl/min. (A) Representative sensorgrams obtained with 4 μM EpoFc monomer and 4 μM EpoFc/IHH are shown. All sensorgrams were zero-adjusted and reference cell data subtracted. (B) Response units at equilibrium are plotted as a function of the log of IgG1 or EpoFc fusion protein concentrations. (C) Binding affinities were derived from the data in B by using biaevaluation software.