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. 2016 Jan 11;6:19063. doi: 10.1038/srep19063

Figure 7. Hsp40 knockdown impairs IAV NP-importin alpha interaction during IAV infection.

Figure 7

(a,b) Hsp40 inhibition significantly reduced NP-importin alpha interaction. A549 cells were transfected with Hsp40 specific siRNA or control siRNA for 24 h (a) or cells were pre-treated with DMSO or KNK437 for 6 h (b). Cells were infected with IAV at MOI = 10 for 4 h followed by IP using anti-NP antibody. Hsp40 and importin-alpha levels were checked in immunoprecipitate by western blotting. (c) Proposed model of NP nuclear import. NP component of incoming vRNPs associates with Hsp40, along with importin alpha and the entire complex is then translocated to the nucleus. Hsp40 inhibition using chemical inhibitor KNK437 or specific siRNA inhibits NP-importin alpha association and hence reduces IAV NP nuclear translocation.