Table 4. Nestedness of Acanthamoeba and Myxomycetes OTUs per region and per site. The sites are in rows and the OTUs in columns. Nestedness (p-value < 0.05) is indicated by numbers in bold.

	Nestedness								Matrix characteristics
	NODF	Z-score	NODF/row	Z-score	NODF/col	Z-score	BR	Z-score	OTUs (col)	Plots (rows)	Occurrences	% of presence
A Acanthamoeba
All	46	48	61	63	38	34	1,710	−29	184	136	4,599	18
AEG	76	16	80	15	76	16	171	−22	85	29	1,097	45
HEG	73	14	82	18	72	13	229	−22	95	36	1,718	50
SEG	66	15	74	18	65	13	276	−15	80	37	1,211	41
B Myxomycetes
All	26	37	43	55	19	22	1,101	−15	203	130	2,225	8
AEG	34	8	49	14	33	6	230	−9	91	33	580	19
HEG	33	9	50	16	32	7	257	−10	105	35	668	18
SEG	42	13	57	22	40	12	293	−13	117	33	818	21

NODF values are comprized between 0 (no nestedness) and 100 (maximum nestedness), Z values > 1.64 indicate significance at p = 0.05.

BR values increase with nestedness, Z values < 1.64 indicate significance at p = 0.05.

Nestedness among rows = species composition.

Nestedness among columns = species occupancy of sites.