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. 2004 Jun 21;101(26):9792–9797. doi: 10.1073/pnas.0403423101

Fig. 5.

Fig. 5.

Regulation of bfrB. (A) P. aeruginosa PAO1 expressing pvdS-lacZ or bfrB-lacZ was cultured for 12 h in dialyzed tryptic soy broth supplemented with various concentrations (0-300 μM) of iron. Protein extracts were collected, and β-galactosidase activity was measured. (B) RNase protection assay. PAO1 and the C6 fur- mutant were grown in iron-limiting and iron-replete media. RNA was isolated and probed with a bfrB-specific probe. (C) Northern blot for BfrB and PrrF RNA isolated from PAO1 and ΔF1-F2 after addition of 2,2′-dipyridyl as for Fig. 3. (D) P. aeruginosa PAO1 (white) and ΔF1-F2 (gray) containing pPZ-bfrB (BfrB::LacZ translational fusion) were grown in various concentrations of iron (0-300 μM) and analyzed for β-galactosidase activity.