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. 2016 Jan 11;6:19118. doi: 10.1038/srep19118

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(a,b) Recombinant FL TDP-43-FLAG proteins were incubated with equimolar amounts of various fragments, including RRM1 (aa103–183), RRM2 (160–295), shorter NTF (1–183), longer NTF (1–295), and CTF35 as indicated by open arrowheads. Proteins were denatured immediately after mixing, or after a 7-day incubation at 4 °C. (a) Coomassie staining. (b) a Western blot using anti-FLAG antibody.