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. Author manuscript; available in PMC: 2016 Jan 11.
Published in final edited form as: J Lab Autom. 2014 Nov 25;20(2):146–153. doi: 10.1177/2211068214560609

Figure 2.

Figure 2

(A) KOALA operation entails pressing a lid, containing micro-wells with reagents and an absorbent pad, onto a base, containing microchannels. When the microfluidic post contacts the fluid, flow is initiated by capillary action. Once the channel is full, an absorbant pad sustains the flow until the well is empty. Once empty, the fluid pins at the entrance of the channel and an air bubble forms in the channel exit. This bubble breaks the fluidic connection between the channel and the absorbent pad – assuring that the channel is not emptied – and completes the fluid transfer. (B) An image of the KOALA-HD pre-filled lid and base, and the resultant base after application of the lid. (C) KOALA-HD compresses 60 assays into the foot print of a traditional glass slide. Lids house micro-wells and absorbent pads, while the bases consist of staggered microchannels. A post is located at both ends of the microchannel. A dimensioned drawing is available in the supplementary information.