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. 2015 Apr 29;2:15006. doi: 10.1038/mto.2015.6

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Copyright © 2015 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Relative biological potency of mouse versus human IL-12. Human PBMCs were incubated with increasing amounts of IL-12 produced from Ad5-yCD/mutTK_SR39rep-mIL12 (expressing mouse IL-12) or Ad5-yCD/mutTK_SR39rep-hIL12 (expressing human IL-12) and IFN-γ production (left) and cell proliferation (right) were measured 72 hours later. For human IL-12, both laboratory grade and clinical grade adenovirus was examined. Each data point represents the mean of three independent determinations with SD <5%. IFN-γ production with no added IL-12 was 3.2 pg/ml.