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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Funct Foods. 2015 Dec 1;19(Pt A):278–287. doi: 10.1016/j.jff.2015.09.035

Figure 4.

Figure 4

Inhibitory effects of 4DN on LPS-induced NF-κB activation in RAW 264.7 macrophages. The cells were seeded in 10-cm dishes for 24 hours, and then treated with LPS or LPS plus 4DN at concentrations indicated in the figure. After 24 hours of incubation, cytoplasmic and nuclear fractions were prepared for immunblotting as described in Methods. The numbers underneath of the blots represent band intensity that was normalized to PARP (nuclear fraction) or β-actin (cytosolic fraction) and measured by Image J software (means of three independent experiments). The standard deviations (all within ±15% of the means) were not shown. PARP and β-Actin were served as an equal loading control for nuclear fraction and cytosolic fraction, respectively. *indicates statistical significance from the LPS-treated group (p < 0.05, n = 3).