Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Funct Foods. 2015 Dec 1;19(Pt A):278–287. doi: 10.1016/j.jff.2015.09.035

Figure 6.

Figure 6

Inhibitory effects of 4DN on phosphorylation of PI3K, Akt and JNK in RAW 264.7 macrophages. The cells were seeded in 10-cm dishes for 24 hours, and then treated with LPS or LPS plus 4DN at concentrations indicated in the figure. After 24 hours of incubation, cells were prepared for immunoblotting as described in Methods. The numbers underneath of the blots represent band intensity that was normalized β-actin and measured by Image J software (means of three independent experiments). The standard deviations (all within ±15% of the means) were not shown. β-Actin were served as an equal loading control. *indicates statistical significance from the LPS-treated group (p < 0.05, n = 3).