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. 2004 May 24;101(26):9879–9884. doi: 10.1073/pnas.0306154101

Fig. 5.

Fig. 5.

Overexpression of Rab17 and mRab17 in Arabidopsis thaliana. (A Top) Western blot of protein extracts from leaves from Arabidopsis transgenic plants using the anti-Rab17 antibody. Samples correspond to lines overexpressing Rab17 protein (L1 to L3), mRab17 protein (L4 and L5), and the nontransgenic control (L6). (Bottom) 2D electrophoresis and immunodetection of Rab17 in protein extracts from line L1 (A) and line L4 (C). (A, B, and D) Analysis of the same samples after dephosphorylation with alkaline phosphatase. The black arrow indicates the position of phosphorylated Rab17, and the open arrow indicates the nonphosphorylated or dephosphorylated Rab17. (B Left) Germination assays of seeds from lines L1 to L5 and nontransgenic control seeds after 11 days of germination in plates containing 100 mM NaCl. (Right) Differences in radicle emergence (days 4-6 of germination) and cotyledon expansion (day 11 of germination) in Rab17 and mRab17 transgenic lines.