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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Dev Cell. 2015 Dec 21;35(6):698–712. doi: 10.1016/j.devcel.2015.11.022

Figure 3. Deletion of TRIM9 increases growth cone size and filopodia density and alters VASP localization to filopodia tips.

Figure 3

A-F) Images and quantification of axonal growth cones from control and netrin-treated TRIM9+/+ and TRIM9−/− neurons, stained for phalloidin (red, left), VASP (green, middle) and βIII tubulin (blue, merge). Quantification of B) growth cone area +/−SEM, C) growth cone filopodia number +/−SEM, D) density of growth cone filopodia +/−SEM, E) filopodia length +/−SEM, and F) VASP fluorescence intensity relative to phalloidin +/− 95% CI from the tip of filopodia into growth cone. G-I) Images and quantification of TRIM9−/− growth cones stained for VASP (red), phalloidin (blue), and Myc (green, Myc or MycTRIM9). H) VASP fluorescence intensity normalized to phalloidin +/− 95% CI from filopodia tip into growth cone. Expression of TRIM9 rescues VASP localization. I) MycTRIM9 fluorescence intensity normalized to phalloidin +/− 95% CI from filopodia tip into growth cone. (See also FigS3)