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. Author manuscript; available in PMC: 2016 Dec 21.
Published in final edited form as: Dev Cell. 2015 Dec 21;35(6):698–712. doi: 10.1016/j.devcel.2015.11.022

Figure 6. Ubiquitination of VASP reduces filopodia lifetime and the rate of VASP dissociation from filopodia tips.

Figure 6

A) Example kymographs of axonal growth cone filopodia from TRIM9+/+ and TRIM9−/− neurons expressing mCherry. B) Filopodial lifetimes +/−SEM in control, netrin, and PR-619 treated TRIM9+/+ and TRIM9−/− neurons and TRIM9+/+ neurons expressing GFP-VASP K-R. C) Cumulative fraction plot of filopodial lifetime demonstrating intermediate phenotype of VASP K-R expressing neurons. D) Image montage of GFP-VASP FRAP at a filopodium tip. Bleach denoted by dashed region, time before and after bleaching in seconds. E) Example of fluorescence intensity data fit to a single exponential (red line), depicting percent fluorescence recovery and t1/2 of fluorescence recovery. F) Fluorescence recovery halftime (t1/2) +/−SEM and G) mean % fluorescence recovery +/−SEM for indicated conditions. (See also Movies S4, S5, FigS6). VASP K-R was expressed in TRIM9+/+ neurons. (See also FigS6)