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Relative expression levels of immunity- and stress-related genes in naive Tribolium castaneum eggs. RNA was isolated from pooled 50 mg samples of eggs laid by parents fed on bacterial diets. Expression levels are presented relative to eggs from non-supplemented diet and normalized against the endogenous housekeeping gene Rps3. The data represent means (±s.d.) of three independent biological replicates (one-way ANOVA, Holm–Sidak, ***p < 0.001).
