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. 2016 Jan 11;16:9. doi: 10.1186/s12885-015-1990-6

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© Vandewynckel et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — PlGF inhibition reduces intratumour hypoxia in experimental HCC. a Immunostaining for pimonidazole in mouse livers following the indicated treatment. Arrows indicate tumours. Scale bars: 100 μm. b Quantification of the immunostaining for pimonidazole. c Lysates of control liver tissue or isolated DEN-induced tumours were subjected to Western blotting for detection of pimonidazole adducts (Pimo). Blotting of β-actin is shown as a loading control. d Densitometry analysis of the pimonidazole blot in (c). e Real-time PCR analysis of Glut1 and Pfk mRNA levels in tumour tissues. IgG = 25w DEN + 5w IgG, aPlGF = 25w DEN + 5w aPlGF. Data are presented as the means ± SD. *p < 0.05