Table 1. Number of unique clones and polymorphism levels in PstI-based DArT libraries differing in the enzyme used for codigestion.

	Estimated no. of unique clones
Codigesting enzyme	Empirical*	Rice†	Hordeum plus Triticum†	Polymorphism level, %‡
AluI	3,100	12,000	18,000	7.0
ApoI	4,900	78,000	88,000	6.9
BanII	16,500	130,000	122,000	3.4
Bsp 12861	6,600	77,000	61,000	2.9
BstNI	5,800	80,000	61,000	10.0
HaeIII	2,000	46,000	27,000	3.5
MseI	4,100	42,000	36,000	4.0
RsaI	3,600	42,000	43,000	8.6
TaqI	3,000	50,000	52,000	10.4

^*See Materials and Methods for a description of procedures.

^†The numbers shown were obtained by in silico analysis (see Materials and Methods) based on bacterial artificial chromosome (BAC) sequences extrapolated from a random set of 327 BAC clones of rice (39 Mbp in total) to the whole genome or from a mixed set of Hordeum and Triticum BAC clones (1.6 Mbp in total) to the whole genome.

^‡Percentage of clones polymorphic between cvs. Clipper and Sahara.