Table 1.

Morphological characterization of spleen dendritic and myeloid subsets.

Sorted subset	Subset classification	Expta	No. of cells	% cells with known morphologyb
				Eosinophil	Neutrophil	Monocyte-like	Dendritic-like
CD11bhiCD11c−Ly6ChiLy6G−CD43+Siglec-F−	Inflammatory monocytes	I	72	1.4	–	97.2c	1.4
		II	68	1.5	–	86.8	11.8
CD11bhiCD11c−Ly6CloLy6G−CD43hiSiglec-F+	Eosinophils	I	72	100	–	–	–
		II	72	100	–	–	–
CD11bhiCD11c−Ly6CloLy6G+CD43+Siglec-F−	Neutrophils	I	26	–	100	–	–
		II	18	–	100	–	–
CD11bhiCD11cloLy6CloLy6G−CD43+/hiSiglec-F−	Resident monocytes	I	76	–	–	68.4	31.6
		II	80	–	–	62.5	37.5
CD11bhiCD11cloLy6C−Ly6G−CD43+Siglec-F−	L-DC	I	78	10.3	–	42.3	47.4
		II	84	6.0	–	32.1	62
CD11b−CD11chiLy6C−Ly6G−CD43−Siglec-F−	CD8+ cDC	I	41	–	–	26.8	73.2
		II	34	–	–	17.7	82.4
CD11b+CD11chiLy6C−Ly6G−CD43−Siglec-F−	CD8− cDC	I	61	–	–	13.1	86.9
		II	59	1.7	–	15.3	83.1

^aData are shown for two independent sorting experiments.

^bCells were cytospun and stained with Giemsa for microscopic classification.

^cDominant subsets are shown in bold.