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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Anal Chem. 2015 Jul 24;87(15):7555–7558. doi: 10.1021/acs.analchem.5b02233

Figure 1.

Figure 1

Multiplexed detection using time-resolved lanthanide-based resonance energy transfer (TR-LRET) and fluorophore conjugated peptide biosensors. (A) Emission spectrum of phosphopeptide-Tb3+ complex (black), excitation (dashed lines) and emission (solid lines) spectra of the two acceptor fluorophores 5-FAM (green) and Cy5 (red). Schematic illustrating TR-LRET detection of Lyn (B) and Syk (C) tyrosine kinase activities using the 5-FAM-SFAStide-A (5-FAM-Ahx-GGEEDEDIYEELDEPGGKbiotinGG) and SAStide-Cy5 (GGDEEDYEEPDEPGGCCy5GG) biosensors respectively.