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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Anal Chem. 2015 Jul 24;87(15):7555–7558. doi: 10.1021/acs.analchem.5b02233

Figure 2.

Figure 2

Time-Resolved Lanthanide-based Resonance Energy Transfer (TR-LRET) detection of phosphorylation-dependent signals and fluorescence cross-interference. (A) Time-resolved luminescence emission spectra for SAStide-Cy5 (dashed line) and pSAStide-Cy5 (solid line). (B) 5-FAM-SFAStide-A (dashed line) and 5-FAM-pSFAStide-A (solid line). Spectra were collected from 15 µM peptide in the presence of 100 µM Tb3+ in 10 mM HEPES, 100 mM NaCl, pH 7.5, λex = 266 nm, in 50 µL total volume, 1 ms collection time, 50 µs delay time, and sensitivity 180. Data represent the average of experiments performed in triplicate.