Table 1.
Name | Kinase | Sequence |
---|---|---|
5-FAM-SFAStide-A | Src-family | 5-FAM-Ahx-GGEEDEDIYEELDEPGGKbGG |
SAStide-Cy5 | Syk | GGDEEDYEEPDEPGGCCy5GG |
5-FAM=5-carboxyfluorescein; Ahx=6-aminohexanoic acid; Kb=biotinyl-L-lysine; CCy5=cysteine thiol conjugated with Cy5.
Sequence segments represented in bold are the core kinase recognition/Tb3+-chelation residues of the biosensor.