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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Anal Chem. 2015 Jul 24;87(15):7555–7558. doi: 10.1021/acs.analchem.5b02233

Table 1.

Peptide biosensor sequences[a][b]

Name Kinase Sequence
5-FAM-SFAStide-A Src-family 5-FAM-Ahx-GGEEDEDIYEELDEPGGKbGG
SAStide-Cy5 Syk GGDEEDYEEPDEPGGCCy5GG
[a]

5-FAM=5-carboxyfluorescein; Ahx=6-aminohexanoic acid; Kb=biotinyl-L-lysine; CCy5=cysteine thiol conjugated with Cy5.

[b]

Sequence segments represented in bold are the core kinase recognition/Tb3+-chelation residues of the biosensor.