Figure 2.

Csf1^op/op mice lack macrophages in the gastric muscularis propria. (A) Resident macrophages in the muscularis propria of wild-type (WT) Csf1^+/+ mice. Whole-mount preparations from the murine stomach were labeled with the F4/80 antibody. Csf1^op/op mice lack macrophages in the gastric muscularis propria when (B) not diabetic and (C) 8 weeks after the development of diabetes. Scale bar: 50 μm for each image. Age of mice: 20–22 weeks. (D) Weight of WT Csf1^+/+ and Csf1^op/op (Op/Op) mice before the development of diabetes. The circles represent individual animals. Csf1^+/+ (WT) were heavier than Csf1^op/op (Op/Op) at the beginning of the experiment. Data are represented as mean ± standard error of the mean. ^aP < .05, unpaired t test. Age of mice: 12–14 weeks.