Figure 6.

Interstitial cells of Cajal (ICC) networks were not affected by diabetes in Csf1^op/opmice while the development of delayed gastric emptying (GE) in diabetic Csf1^+/+was associated with reduced density of ICC. Images of Kit immunoreactivity in the muscularis propria of the distal corpus of the mouse stomach in nondiabetic Csf1^+/+ (A) wild type (WT) and Csf1^op/op (B) Op/Op; in diabetic Csf1^+/+ (C) and Csf1^op/op (D) with normal GE; and in diabetic Csf1^+/+ with delayed GE (E) (age-matched/representative of n = 3). Scale bar: 100 μm for each image. Whiskers show the mean ± standard deviation of the scores from biological replicates (n = 3 mice; P < .05, one-way analysis of variance with Dunnett’s post test). Each circle represents the overall score from nine regions in each individual animal. Age of mice: 20–22 weeks for diabetic and nondiabetic Csf1^op/op mice; 20–22 weeks for WT control; diabetic Csf1^+/+ (WT) mice with delayed or normal GE (14–20 weeks old).