Figure 4.

Confocal micrographs of excised, structurally conserved collateral vessels reveal an increase in vessel diameter in ligated tissue when compared to paired sham tissue. A.) Excised collateral vessel stained with α-smooth muscle actin with feeding vessel, 1^st branch, and 2^nd branch identified. B.) Individual diameter measurements for sham tissues were paired with corresponding diameter measurements for ligated tissues (in the same mouse) and a paired t-test was run (p-value < 0.01). Five mice were used to quantify collateral vessel diameters. Scale bar = 50 µm.