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. 2015 Dec 22;35(6):e00276. doi: 10.1042/BSR20150165

Figure 4. Apigenin induces a caspase-dependent apoptosis in BT-474 cells.

Figure 4

(A) Apigenin induces apoptosis via a caspase-dependent apoptosis pathway in BT-474 cells. BT-474 cells were treated with apigenin (0–60 μM) for 24 h. Whole-cell lysates were analysed by western blotting with anti-FAS, anti-cleaved caspase-8, anti-caspase-3, anti-cleaved caspase-3, anti-PARP and anti-tubulin antibodies. The data shown are representative of three independent experiments that gave similar results. (B) Effect of caspase-8 and caspase-9 inhibitors on apigenin-induced apoptosis in BT-474 cells. BT-474 cells were exposed to 60 μM apigenin with or without the caspase-8 inhibitor (40 μM) or the caspase-9 inhibitor (40 μM) for 24 h, the cell lysates were separated by SDS/PAGE, and western blotting with specific antibodies was performed (anti-cleaved caspase-8, anti-cleaved caspase-3, anti-PARP and anti-tubulin). The data shown are representative of three independent experiments that gave similar results. (C) Effect of caspase-8 and caspase-9 inhibitors on BT-474 cell proliferation. BT-474 cells were exposed to 60 μM apigenin with or without the caspase-8 inhibitor (40 μM) or the caspase-9 inhibitor (40 μM) for 72 h and photographed by phase-contrast microscopy (original magnification, ×40).