Fig. 2. : modulatory effect of Trypanosoma cruzi in cellular metabolism of H9c2-infected cells. A: quantitative polymerase chain reaction analyses of Pten mRNA; B: Western blots analyses and quantification of PTEN, phospho-PTEN (P-PTEN), and the reference protein β-actin; C: miRNAs expression patterns in T. cruzi-infected cells. The presented values are the average from three independent experiments and the error bars represent the standard deviation of the mean. One-way analysis of variance testing showed significant differences between the control and cell samples and the significance level was set at p < 0.05 (***).