Fig. 1. : co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) enhances human immunodeficiency virus-1 subtype B conserved peptides (HIVBr18)-induced T-cell responses. Two weeks after the last immunisation with HIVBr18 co-administrated with pVAX1 or pGM-CSF, pooled spleen cells from six BALB/c mice were cultured in the presence of both pooled and individual HIVBr18-encoded peptides (5 µM), or medium only. Frequencies of interferon (IFN)-γ-secreting T-cells against pooled (A) and individual (B) HIVBr18-encoded peptides were measured by ELISPOT and shown as spot forming units (SFU) per 106 cells. Mean plus standard deviation of three independent experiments are shown in A. One representative experiment of three is shown in B. Only peptides frequently recognised are shown. Dotted line represents ELISPOT cut-off (15 SFU/106 cells). Asterisks mean p < 0.001.