Fig. 2. : sensitivity of different polymerase chain reaction (PCR) primer assays. A-C: 10-fold DNA serial dilutions from a Leishmania donovani pure culture were used in PCR reactions with different primer sets as indicated; A: JW 11/12 KDNA PCR (Rodgers et al. 1990) [Lane 1: 100 bp DNA ladder; L2-10: 10-fold descending serial dilutions of Leishmania DNA from 100 ng-1 fg; 11: -ve control (no DNA)]; B: LdF/LdR KDNA PCR (Salotra et al. 2001) [Lane 1: -ve control (no DNA); 2-11: 10-fold ascending serial dilutions ofLeishmania DNA from 0.1 fg-100 ng; 12: 100 bp DNA ladder]; C: LITSR/L5.8S internal transcribed spacer 1 PCR (El Tai et al. 2000) [Lane 1: -ve control (no DNA); 2: 100 bp DNA ladder; 3-11: 10-fold descending serial dilutions of Leishmania DNA from 100 ng-1 fg].
