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. 2015 Dec;110(8):1017–1023. doi: 10.1590/0074-02760150286

Fig. 4. : specificity assays of polymerase chain reaction (PCR). A:Leishmania LdF/LdR KDNA PCR in the presence ofMycobacterium tuberculosis DNA [Lane 1: 100 bp DNA ladder; 2: cutaneous leishmaniasis (CL) sample 35; 3:Leishmania +ve control (CL culture); 4: -ve control (no DNA); 5: CL sample 34; 6: CL sample 37; 7: CL sample 36; 8-10:M. tuberculosis samples (n = 3/5)]; B:Leishmania JW11/12 KDNA PCR in the presence of human, Mycobacterium leprae and M. tuberculosis DNA [Lane 1: -ve control (no DNA); 2: 100 bp DNA ladder; 3: +ve control (CL culture); 4-6: M. lepraeand human DNA samples (n = 3/5); 7-8: M. tuberculosisDNA samples (n = 2/5)]; C: LITSR/L5.8S internal transcribed spacer 1 PCR in the presence of human, M. leprae and M. tuberculosis DNA [Lane 1: -ve control (no DNA); 2: 100 bp DNA ladder; 3-5: M. leprae and human DNA samples (n = 3/5); 6-7: M. tuberculosis DNA samples (n = 2/5); 8: CL sample 32 (repeat); 9: CL sample 36; 10: CL sample 37 (repeat); 11: CL sample 35 (repeat); 12: +ve control (CL culture)].

Fig. 4