| KDNA genus Leishmania specific
PCR (Rodgers et al. 1990) |
JW11 (forward):
5’-CCTATTTTACACCAACCCCCAGT-3’ JW12 (reverse):
5’-GGGTAGGGGCGTTCTGCGAAA-3’ |
Initial denaturation at 94°C for
1 min followed by 34 cycles of denaturation at 94°C for 30 s, annealing
at 58°C for 30 s, and extension at 72°C for 30 s |
| KDNA Leishmania donovani
species-specific PCR (Salotra et al. 2001) |
LdF (forward):
5’-AAATCGGCTCCGAGGCGGGAAAC-3’ LdR (reverse):
5’-GGTACACTCTATCAGTAGCAC-3’ |
Initial denaturation at 94°C for
2 min followed by 40 cycles of denaturation at 94°C for 1 min, annealing
at 45°C for 1 min, and extension at 72°C for 2 min, with a final
extension at 72°C for 3 min |
| Internal transcribed spacer 1 PCR
genusLeishmania specific (El Tai et al. 2000) |
LITSR (forward):
5’-CTGGATCATTTTCCGATG-3’ L 5.8S (reverse):
5’-TGATACCACTTATCGCACTT-3’ |
Initial denaturation at 95°C for
2 min, followed by 34 cycles of denaturation at 95°C for 20 s, annealing
at 53°C for 30 s, and extension at 72°C for 1 min, with a final extension
of 72°C for 6 min |
