Table 1. Macromolecule-production information for TvTPS1.

Source organism	T. vulgaris
DNA source	cDNA derived from T. vulgaris mRNA
Forward primer†	ATGGCTACCCTTAGCATGCAAGTGTCC
Reverse primer†	CACATATGGCTCGAAAATAAGGCCTCCC
Forward primer‡	CACCATGGCTACCCTTAGCATGCAAGTGTCC
Reverse primer‡	CTACACATATGGCTCGAAAATAAGGCCTCCC
Cloning vector	pCR 2.1-TOPO
Expression vector	pENTR-D-TOPO, Champion pET300/NT-DEST
Expression host	E. coli BL21 CodonPlus
Complete amino-acid sequence of the construct produced	MHHHHHHITSLYKKAGSMRRSGNYQAPVWNNDFIQSFSTDKYKDEKFLKKKEELIAQVKVLLNTKMEAVKQLELIEDLRNLGLTYYFEDEFKKILTSIYNEHKGFKNEQVGDLYFTSLAFRLLRLHGFDVSEDVFNFFKNEDGSDFKASLGENTKDVLELYEASFLIRVGEVTLEQARVFSTKILEKKVEEGIKDEKLLAWIQHSLALPLHWRIQRLEARWFLDAYKARKDMNPIIYELGKIDFHIIQETQLQEVQEVSQWWTNTNLAEKLPFVRDRIVECYFWALGLFEPHEYGYQRKMAAIIITFVTIIDDVYDVYDTLDELQLFTDAIRKWDVESISTLPYYMQVCYLAVFTYASELAYDILKDQGFNSISYLQRSWLSLVEGFFQEAKWYYAGYTPTLAEYLENAKVSISSPTIISQVYFTLPNSTERTVVENVFGYHNILYLSGMILRLADDLGTTQFELKRGDVQKAIQCYMNDNNATEEEGTEHVKYLLREAWQEMNSAMADPDCPLSEDLVFAAANLGRTSQFIYLDGDGHGVQHSEIHNQMGGLIFEPYV

^†Used in TvTPS cDNA cloning.

^‡Used in cloning into expression vector.