Table 2. Crystallization.

Method	Sitting drop
Plate type	96-well
Temperature (K)	292
Protein concentration (mg ml−1)	10
Buffer composition of protein solution	20 mM Tris–HCl pH 7.8, 1 mM MgCl2, 4 mM DTT
Composition of reservoir solution	0.2 M ammonium tartrate dibasic pH 7.0, 20%(w/v) polyethylene glycol 3350
Volume and ratio of drop	0.4–0.6 µl, 1:1 and 1:2
Volume of reservoir (µl)	70